rabbit anti igfbp5 polyclonal antibody  (Boster Bio)

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Increasing chi-miR-3031 and siRNA-IGFBP5 levels notably decreased IGFBP5 mRNA expression. a : Effect of chi-miR-3031 on IGFBP5 mRNA. b : Effect of siRNA-IGFBP5 on IGFBP5 mRNA. P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Expressing, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Effects of chi-miR-3031 and siRNA-IGFBP5 on IGFBP5 protein levels. a : Western blot analysis results. b : Densitometric quantification of western blot results. Protein levels were normalized to β-actin. P < 0.05 is shown as *, and P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Western Blot, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Protein expression levels of κ-casein ( a ) and β-casein ( b ) in GMECs transfected with MC, NC and siRNA-IGFBP5. P < 0.05 is shown as *, and P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Expressing, Transfection, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Effects of chi-miR-3031 and siRNA-IGFBP5 on p-mTOR protein levels. a : Western blot analysis results. b : Densitometric quantification of western blot results. P < 0.05 is shown as *, and P < 0.01 is shown as **. MC: Chi-miR-3031 mimic. NC: Negative control

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Western Blot, Negative Control

Journal: BMC Veterinary Research

Article Title: Chi-miR-3031 regulates beta-casein via the PI3K/AKT-mTOR signaling pathway in goat mammary epithelial cells (GMECs)

doi: 10.1186/s12917-018-1695-6

Figure Lengend Snippet: Primer information for RT-qPCR

Article Snippet: Subsequently, the membranes were incubated overnight at 4 °C with a rabbit anti-IGFBP5 polyclonal antibody (1:300, Boster, USA), a rabbit anti-mTOR polyclonal antibody (1:100, Boster, USA), a rabbit anti-p-mTOR polyclonal antibody (1:100, Boster, USA) or a mouse β-actin monoclonal primary antibody (1:1000, Beyotime, Jiangsu, China).

Techniques: Sequencing

Journal: Molecular Medicine Reports

Article Title: Critical role of insulin-like growth factor binding protein-5 in methamphetamine-induced apoptosis in cardiomyocytes

doi: 10.3892/mmr.2014.2572

Figure Lengend Snippet: The mRNA and protein expression of IGFBP5 increases in a dose-dependent manner in NRVMs treated with MA for 48 h. NRVMs were incubated with MA at varying concentrations (0, 0.5, 1.0 or 1.5 mM). (A) Total RNA was subjected to reverse transcription polymerase chain reaction. (B) Whole cell lysates were analyzed by western blotting. An antibody against β-actin was used as the loading control. The data are presented as the mean ± standard deviation (n = 6; * P<0.01). MA, methamphetamine; NRVMs, neonatal rat ventricular myocytes; IGFBP5, insulin-like growth factor binding protein-5.

Article Snippet: Non-specific binding was blocked with 5% non-fat skim milk in Tris-buffered saline with Tween 20 (TBST) at room temperature for 1 h. Membranes were incubated with rabbit polyclonal anti-IGFBP5 antibody (1:1,000; Abcam, Cambridge, UK) and rabbit polyclonal anti-caspase-3 antibody (1:200, Santa Cruz Biotechnology, Inc.) at 4°C overnight.

Techniques: Expressing, Incubation, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Control, Standard Deviation, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Critical role of insulin-like growth factor binding protein-5 in methamphetamine-induced apoptosis in cardiomyocytes

doi: 10.3892/mmr.2014.2572

Figure Lengend Snippet: siIGFBP5 silences the protein expression of IGFBP5 in NRVMs. (A) Nonspecific silencing control lane showed no effect of siNC in NRVMs 48 h post-transfection. siIGFBP5-1 and siIGFBP5-2 demonstrated efficient knockdown of IGFBP5 at the protein level. (B) The knockdown efficacy of siIGFBP5 was not affected following treatment with MA. An antibody against β-actin was used as the loading control. The data are presented as the mean ± standard deviation (n = 6; * P<0.01, ** P>0.05). MA, methamphetamine; NRVMs, neonatal rat ventricular myocytes; IGFBP5, insulin-like growth factor binding protein-5; si, small interfering.

Article Snippet: Non-specific binding was blocked with 5% non-fat skim milk in Tris-buffered saline with Tween 20 (TBST) at room temperature for 1 h. Membranes were incubated with rabbit polyclonal anti-IGFBP5 antibody (1:1,000; Abcam, Cambridge, UK) and rabbit polyclonal anti-caspase-3 antibody (1:200, Santa Cruz Biotechnology, Inc.) at 4°C overnight.

Techniques: Expressing, Control, Transfection, Knockdown, Standard Deviation, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Critical role of insulin-like growth factor binding protein-5 in methamphetamine-induced apoptosis in cardiomyocytes

doi: 10.3892/mmr.2014.2572

Figure Lengend Snippet: Effect of siIGFBP5 silencing of the IGFBP5 gene on MA-induced apoptosis evaluated by TUNEL staining. (A) Effects of suppressing the IGFBP5 gene in 1.5 mM MA-treated NRVMs assessed by TUNEL assay. The 0 mM + siNC group was pretreated with nonspecific silencing control without MA as the control group. The 1.5 mM + siIGFBP5-1 group and the 1.5 mM + siIGFBP5-2 group were pretreated with two sequences (siIGFBP5-1 and siIGFBP5-2) that target IGFBP5, respectively. Nuclei were counterstained with DAPI (blue). Apoptotic cells were stained with TUNEL (green). (B) Quantification of the percentage of apoptotic cells using a standard cell counting method with the TUNEL assay. The apoptotic rate was calculated as follows: (number of apoptotic cells / total number of cells) × 100%. The data are presented as the mean ± standard deviation from three independent experiments (n = 5; * P<0.01, ** P>0.05). MA, methamphetamine; IGFBP5, insulin-like growth factor binding protein-5; TUNEL, terminal deoxyribonucleotide transferase-mediated dUTP nick end-labeling; DAPI, 4′,6′-diamidino-2-phenylindole; NRVMs, neonatal rat ventricular myocytes; si, small interfering.

Article Snippet: Non-specific binding was blocked with 5% non-fat skim milk in Tris-buffered saline with Tween 20 (TBST) at room temperature for 1 h. Membranes were incubated with rabbit polyclonal anti-IGFBP5 antibody (1:1,000; Abcam, Cambridge, UK) and rabbit polyclonal anti-caspase-3 antibody (1:200, Santa Cruz Biotechnology, Inc.) at 4°C overnight.

Techniques: TUNEL Assay, Staining, Control, Cell Counting, Standard Deviation, Binding Assay, End Labeling

Journal: Molecular Medicine Reports

Article Title: Critical role of insulin-like growth factor binding protein-5 in methamphetamine-induced apoptosis in cardiomyocytes

doi: 10.3892/mmr.2014.2572

Figure Lengend Snippet: Expression of caspase-3 was determined by western blot analysis. The intensity of each band was quantified by densitometry and the data were normalized using the β-actin signal. The 0 mM + siNC group (control group) was considered the basal level and the other groups were expressed as fold changes compared with the control. The data are presented as the mean ± standard deviation (n = 6; * P<0.01, ** P>0.05). IGFBP5, insulin-like growth factor binding protein-5; si, small interfering.

Article Snippet: Non-specific binding was blocked with 5% non-fat skim milk in Tris-buffered saline with Tween 20 (TBST) at room temperature for 1 h. Membranes were incubated with rabbit polyclonal anti-IGFBP5 antibody (1:1,000; Abcam, Cambridge, UK) and rabbit polyclonal anti-caspase-3 antibody (1:200, Santa Cruz Biotechnology, Inc.) at 4°C overnight.

Techniques: Expressing, Western Blot, Control, Standard Deviation, Binding Assay

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Expressions of IGFBP-5, cFLIP in cervical intraepithelial neoplasia, cervical carcinoma and their clinical significances: a molecular pathology

doi: 10.1186/1756-9966-28-70

Figure Lengend Snippet: The relationship between expression of IGFBP-5 and cFLIP and clinicopathological parameters in CC

Article Snippet: Primary antibodies used in this study include IGFBP-5 rabbit anti-human polyclonal antibody (Boster Co., Ltd., Wuhan) and cFLIP rabbit anti-human polyclonal antibody (American Neomarker Co.).

Techniques: Expressing